Comprehensive evaluation of enzymatic proteolysis for amlyoid reduction in macular amyloidosis by in vitro, in vivo and in silico methods

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Original Article

Author Details : Vrushali Neve*, Vrushali Bhalchim, Pawan Nandkishor Karwa, Ajay Sabu, Swapnil Runje

Volume : 10, Issue : 2, Year : 2024

Article Page : 208-213

https://doi.org/10.18231/j.ijced.2024.037

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Abstract

Introduction: Skin diseases represent a significant burden on global healthcare systems, with protein deposition playing a pivotal role in their pathogenesis, including conditions such as macular amyloidosis. This study aimed to assess the proteolytic activity of the enzyme Papain on deposited protein, offering insights into potential therapeutic strategies.
Materials and Methods: Using a chicken model, the deposition of protein, a key component in skin diseases, was observed predominantly in the ileum. The dose in different concentrations was given to the ileum and observation was taken on alternative days.Dose of complex protein (albumin, beta lactalbumin, alpha-lactalbumin, Immunoglobulin 500mg/ml and 750mg /ml given respectively. Treatment with Papain demonstrated its ability to effectively break down the deposited protein. In invivo method the designated area of the animal's skin where the induction was occur to ensure proper application and observation were Shaved and cleaned. One vehicle control group, one disease group and one test group. The dosing was done by subcutaneous route. Dose of complex protein (albumin, beta lactalbumin, alpha-lactalbumin, Immunoglobulin) 500mg/ml and 750mg /ml were given . For Papaya Extract Dose of 500mg/ml and 750mg /ml were given to animals.
Result: In cheken ilium 5days after dosing, length of patch were observed for five days. In wistar rat after 4days the rats seem to develop hyper pigmented patches which were brown in color in disease group. Then the groups of Animal were injected with papain by SC route. Daily interval observations were done. On the 4th day treatment spot becomes reduced and it disappeared slowly. In silico score observed upto -8 which indicate positive binding affinity.
Conclusion: These findings underscore the potential of Papain as a promising candidate for the management of protein-related skin disorders, highlighting the importance of enzymatic intervention in mitigating their pathological manifestations.
 

Keywords: Skin illness, macular amyloidosis, Papain, inflammation

How to cite : Neve V, Bhalchim V, Karwa P N, Sabu A, Runje S, Comprehensive evaluation of enzymatic proteolysis for amlyoid reduction in macular amyloidosis by in vitro, in vivo and in silico methods. IP Indian J Clin Exp Dermatol 2024;10(2):208-213

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